The KDM3B Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting human KDM3B in the HEK293T cell line. This loss-of-function model facilitates study of the histone demethylase KDM3B, which erases H3K9me1/me2 repressive marks. The polyclonal format provides a heterogeneous pool of edited cells, enabling robust functional analysis without clonal selection.
HEK293T is a human embryonic kidney epithelial line immortalized with adenovirus 5 DNA and expressing SV40 large T-antigen. Renowned for high transfection efficiency and rapid growth, it is widely used for transient protein expression, viral packaging, and CRISPR-based genome editing. Its well-characterized signaling landscape makes it an optimal host for dissecting KDM3B-dependent mechanisms.
KDM3B is a histone lysine demethylase that removes methyl groups from H3K9me1/me2, promoting chromatin relaxation and transcriptional activation. It integrates signals from HIF1A, Wnt ligands, and androgen receptor (AR), and is regulated by MYC, TP53, and TGF-??. Downstream targets include CCND1, MYC, BCL2, NANOG, VEGF, and gluconeogenic enzymes PCK1 and G6PC. KDM3B interacts with the SWI/SNF complex component BRG1 (SMARCA4), AR, HIF1A, Sp1, and Ku70/Ku80. Representative pathway nodes are KDM3B-H3K9me2-??-catenin-TCF/LEF-CCND1, HIF1A-KDM3B-VEGF, and AR-KDM3B-KLK3.
In HEK293T cells, KDM3B knockout leads to accumulation of H3K9me2 and dysregulation of target gene expression. This model is particularly valuable for investigating epigenetic contributions to proliferation, metabolic reprogramming, and hypoxia adaptation. The HEK293T background supports studies of Wnt/??-catenin-driven transcription and AR signaling, relevant to cancer biology and metabolic disorders. The knockout cells also allow exploration of chromatin crosstalk and validation of KDM3B as a therapeutic target.
Applications include functional genomics, drug target validation, and pathway analysis. Assays commonly used with this product are Western blotting for KDM3B and H3K9me2, RT-qPCR and RNA-seq for expression profiling, ChIP-qPCR for H3K9me2 occupancy, flow cytometry for cell cycle and apoptosis, proliferation and colony formation assays, metabolic assays (glucose uptake), luciferase reporters for Wnt/HIF activity, and co-immunoprecipitation for protein interactions. The polyclonal knockout cells are also suitable for high-throughput screening of KDM3B modulators and stem cell differentiation studies. For additional information, please contact Ascent Research.
