The KDM5B Knockout HAP1 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population designed for loss-of-function studies of the KDM5B gene in a near-haploid human cell background. This polyclonal pool carries heterogeneous disruptions of the target locus, providing a robust model for functional genomics and cancer epigenetics research. The product is supplied as a live, polyclonal population suitable for immediate expansion and experimental use.
The host HAP1 cell line is a near-haploid human male chronic myeloid leukemia (CML) line derived from the KBM-7 patient isolate. Its near-haploid karyotype simplifies genetic analysis and enables high-efficiency CRISPR-mediated gene targeting, making it a favored model for genetic screens, drug sensitivity profiling, and mechanistic studies of leukemia-associated pathways. HAP1 cells retain key signaling networks of myeloid leukemia, allowing dissection of gene function in a disease-relevant context.
KDM5B (lysine-specific demethylase 5B) is a histone demethylase that removes methyl groups from di- and trimethylated lysine 4 of histone H3 (H3K4me2/3), acting as a transcriptional repressor. It functions within a network regulated by transcription factors E2F1, RB1, and MYC, and interacts with corepressors including SIN3B, HDAC1, HDAC2, and RBBP4 to modulate chromatin state. KDM5B targets genes such as the HOX cluster and CDKN1A, thereby influencing cell cycle progression, differentiation, and the DNA damage response. In hematopoietic contexts, KDM5B participates in stem cell maintenance and lineage commitment decisions.
In HAP1 cells, disruption of KDM5B provides a powerful system to study its dual roles in oncogenesis. KDM5B can act as a tumor suppressor by repressing proliferation-associated genes or as an oncogene in certain leukemias and solid tumors. This polyclonal knockout model enables investigation of context-dependent functions in a CML background, including effects on proliferation, drug responses, and epigenetic reprogramming. It is particularly suited for dissecting KDM5B??s contribution to histone methylation dynamics and gene expression programs relevant to leukemogenesis.
This polyclonal knockout cell population is designed for a wide array of research applications, including functional genetic screens, epigenetic modifier studies, and drug target validation. Researchers can employ ChIP-qPCR to assess H3K4 methylation levels, RT-qPCR and western blotting to monitor target gene expression, and flow cytometry to evaluate cell cycle perturbations. Additionally, proliferation and drug sensitivity assays can be performed to assess effects on chemoresistance. For specific inquiries or experimental guidance, please contact Ascent Research.
