KDM5C Knockout T47D Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Breast (mammary gland)
Disease:
Ductal carcinoma

Datasheet

This product provides a CRISPR/Cas9-edited polyclonal KDM5C knockout population in T-47D cells, a human breast ductal carcinoma line that expresses estrogen and progesterone receptors and serves as a model of hormone-responsive breast cancer. KDM5C encodes a histone lysine demethylase that removes methyl groups from H3K4me3 and H3K4me2, functioning in transcriptional repression through interactions with the REST corepressor complex, HDAC1/2, SIN3B, CoREST, and LSD1. The knockout model enables detailed investigation of KDM5C's role in estrogen receptor signaling, histone methylation dynamics, and breast tumor progression. Applications include epigenetic drug screening, chromatin immunoprecipitation?CqPCR, proliferation and migration assays, and tamoxifen sensitivity studies, making it a valuable tool for dissecting chromatin regulation and identifying therapeutic vulnerabilities.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

T-47D

Sex of Donor

Female

Age

54 years

Derived From Site

Metastatic; Pleural effusion

Gene Name

KDM5C

Gene Identifier

NCBI Gene ID 8242

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 10μg/mL Insulin, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The KDM5C Knockout T-47D Polyclonal Cells product comprises a CRISPR/Cas9-edited polyclonal population of T-47D cells harboring a targeted disruption of the KDM5C gene, providing a powerful loss-of-function model for investigating this histone demethylase.

This polyclonal format preserves biological variability and avoids artifacts associated with single-cell cloning, making it suitable for robust functional studies in breast cancer research.