The KDM5D Knockout HeLa Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population featuring targeted disruption of the KDM5D gene in the HeLa human cervical adenocarcinoma cell line. This loss-of-function model is generated using CRISPR/Cas9-mediated gene disruption, producing a heterogeneous pool of cells with abrogated KDM5D expression. The product is supplied as viable polyclonal cells suitable for a wide range of downstream functional assays and omics analyses. This knockout cell pool enables researchers to dissect KDM5D-dependent epigenetic mechanisms without the need for single-cell clone isolation.
The host cell line, HeLa, is an immortalized epithelial cell line derived from a cervical carcinoma biopsy and is positive for human papillomavirus type 18 (HPV18). HeLa cells have served as a cornerstone in biomedical research for decades, providing a robust and well-characterized platform for studying cancer biology, drug response, gene regulation, and signal transduction. Their rapid proliferation, ease of culture, and extensive characterization make HeLa cells an ideal host for generating gene knockout models aimed at investigating fundamental cellular processes.
KDM5D encodes a histone lysine demethylase specific for H3K4me2/3, thereby mediating transcriptional repression. This Y-encoded enzyme acts downstream of androgen receptor (AR) and SRY, and interacts with MLL complexes, histone deacetylases, and JARID1 family members to coordinate chromatin remodeling. KDM5D demethylates H3K4me3 at promoters of target genes, including cell cycle and apoptosis regulators, functioning as a transcriptional corepressor within chromatin organization and androgen receptor signaling pathways.
In the HeLa context, disruption of KDM5D provides a model to investigate how loss of this Y-linked demethylase influences chromatin and gene expression in a widely used epithelial cancer line. Although HeLa cells are female-derived, this knockout model enables controlled studies of KDM5D function, such as by ectopic re-expression. Researchers can examine alterations in H3K4 methylation, expression of cell cycle and apoptosis genes, and effects on proliferation and DNA damage response, contributing to understanding its roles in cancer epigenomics and spermatogenesis.
These polyclonal knockout cells are well-suited for diverse applications. Epigenetic analyses can utilize ChIP-qPCR to profile H3K4me3, while western blot and RT-qPCR confirm KDM5D loss and target gene changes. Transcriptome analysis via RNA-seq reveals genome-wide expression alterations. Functional readouts such as proliferation and apoptosis assays delineate biological consequences. These cells enable investigations into sex chromosome biology, drug resistance mechanisms, and functional genomics. For more information, please contact Ascent Research.
