KHDRBS1 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from HeLa cells, featuring disruption of the KHDRBS1 gene. This product provides a heterogeneous pool of cells with targeted gene disruption, enabling loss-of-function studies. The polyclonal format avoids clonal selection biases and offers a robust platform for investigating gene function in a physiologically relevant cellular context.
HeLa cells are an immortalized human epithelial cell line originating from cervical adenocarcinoma of a 31-year-old African American woman. They are HPV18-positive, highly aneuploid, and widely used as a model for cancer biology, cell cycle regulation, and general cell biology. Their robust growth and ease of manipulation make them ideal for gene-editing applications.
KHDRBS1 (Sam68) is an RNA-binding protein that serves as a critical adaptor linking signal transduction to RNA metabolism. It is phosphorylated by Src family kinases and other growth factor receptor-activated pathways, regulating alternative splicing, mRNA stability, and translation. Key downstream targets include CD44 pre-mRNA splicing and Bcl-x alternative splicing, influencing cell cycle progression and apoptosis. KHDRBS1 interacts with Src, Grb2, PLC??1, RNA polymerase II, and heterogeneous nuclear ribonucleoproteins (hnRNPs), integrating signals from cytokines and growth receptors.
In HeLa cells, which harbor activated signaling pathways, disruption of KHDRBS1 provides insight into its role in coupling oncogenic signals to RNA processing. This model is particularly relevant for studying how post-transcriptional regulation contributes to cancer phenotypes such as aberrant splicing of apoptotic regulators and altered cell cycle control. The polyclonal population maintains diversity, allowing assessment of knockout impact without clonal artifacts.
These knockout cells are suitable for investigating signal transduction to RNA metabolism, alternative splicing decisions, and apoptosis regulation. Researchers can employ assays like Western blotting, RT-qPCR for splice isoforms, RNA-seq, immunofluorescence, and co-immunoprecipitation to dissect KHDRBS1 interactions. Functional studies may include flow cytometry for apoptosis, migration assays, and phospho-signaling analysis. This tool is valuable for drug target validation and cancer biology research. For additional details, please contact Ascent Research.
