The KHDRBS3 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human HEK293T cell line, offering a loss-of-function model for the KHDRBS3 gene. This product provides a heterogeneous pool of cells carrying disruptive edits at the KHDRBS3 locus, enabling researchers to study the functional consequences of KHDRBS3 depletion in a well-characterized cellular background. Designed for applications in RNA biology and signal transduction, these polyclonal knockout cells circumvent clonal selection artifacts, providing a robust system for mechanistic and phenotypic analyses.
HEK293T cells are human embryonic kidney cells that constitutively express the SV40 large T antigen, a feature that supports episomal replication of plasmids bearing the SV40 origin of replication. Widely adopted for transfection, viral production, and recombinant protein expression, this host line is derived from the original HEK293 isolate and is highly amenable to genetic manipulation. Its robust growth characteristics and high transfection efficiency make it an ideal platform for generating knockout pools and performing downstream assays that require efficient gene delivery and protein overexpression.
KHDRBS3, also known as T-STAR, is an RNA-binding protein of the STAR family that bridges tyrosine kinase signaling and alternative splicing regulation. It is activated through phosphorylation by Src family kinases, including Fyn and Lck, in response to growth factor receptor stimulation. Upon activation, KHDRBS3 interacts with Sam68 and other KH domain-containing proteins to modulate the alternative splicing of key target transcripts such as CD44 and Bcl-x. This signaling-dependent splicing control directly influences cell proliferation, survival, and differentiation pathways, positioning KHDRBS3 as a critical node in the coordination of extracellular cues and post-transcriptional gene expression.
Establishing the KHDRBS3 knockout in HEK293T cells creates a tractable model for dissecting the molecular interplay between Src kinase signaling and RNA processing. The inherently high transfection competency of HEK293T cells allows for straightforward reconstitution with wild-type or mutant KHDRBS3 constructs, enabling structure?Cfunction studies of its RNA-binding domain and phosphorylation sites. Moreover, the absence of functional KHDRBS3 in this polyclonal background permits unambiguous phenotypic attribution in transcriptome-wide analyses, facilitating the identification of direct splicing targets and signaling effectors in a simplified, yet physiologically relevant, cellular context.
The KHDRBS3 Knockout HEK293T Polyclonal Cells are suited for a wide range of experimental approaches, including RNA-seq to characterize global splicing changes, RT-qPCR to quantify isoform switching of targets like CD44 and Bcl-x, and co-immunoprecipitation to probe interactions with Sam68 and splicing factor complexes. Additional applications encompass phospho-signaling profiling, migration and invasion assays, and reporter-based splicing assays. These cells are particularly valuable for cancer research, including glioblastoma and breast cancer studies, and for validating KHDRBS3 as a therapeutic target. For further information or bulk orders, please contact Ascent Research.
