KHNYN Knockout HT29 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population that enables functional studies of KHNYN in a human colorectal adenocarcinoma background. This polyclonal format ensures a heterogeneous pool of cells with targeted disruptions in the KHNYN gene, facilitating loss-of-function analyses without clonal selection bias. The CRISPR/Cas9-mediated gene disruption creates a reliable model for investigating KHNYN-dependent antiviral and RNA regulatory mechanisms.
The HT29 host cell line originates from a 44-year-old female with colorectal adenocarcinoma and is a well-characterized model of intestinal epithelial cells. These cells can differentiate into enterocyte-like cells, mimicking key aspects of the intestinal epithelium. HT29 cells are extensively used to study epithelial barrier function, mucosal immunity, and host-pathogen interactions, making them an ideal platform for examining innate immune factors such as KHNYN at mucosal surfaces.
KHNYN is an interferon-inducible RNA-binding protein with endonuclease activity, acting as a restriction factor against retroviruses like HIV-1. It binds viral RNA via its KH domain and promotes degradation through its NYN endonuclease domain. Expression is induced by type I interferons (IFN-??/??) through STAT1 and IRF9. KHNYN interacts with HIV-1 capsid and colocalizes with stress granule proteins G3BP1 and TIA1, facilitating viral RNA decay. This protein functions downstream of the RIG-I/MAVS/IRF3 antiviral signaling axis and associates with CNBP, suggesting broader roles in RNA metabolism.
In the context of HT29 cells, KHNYN knockout provides a unique model to dissect cell-intrinsic antiviral defenses in intestinal epithelia. As a primary entry site for viruses, the gut mucosa relies on interferon-induced restriction factors to limit infection. Disrupting KHNYN in these cells allows researchers to evaluate its impact on viral replication, stress granule dynamics, and innate signaling pathways, linking epithelial biology with antiviral immunity.
Applications include studying antiviral innate immunity, HIV restriction, RNA degradation, and stress granule biology. Representative assays include western blotting, RT-qPCR, RNA-seq, co-immunoprecipitation, viral replication assays, immunofluorescence, and flow cytometry. These cells support mechanistic studies of interferon-stimulated gene function and viral-host interactions. For further details, please contact Ascent Research.
