The KIAA0319L Knockout HEK293T Polyclonal Cells product comprises a heterogeneous population of HEK293T cells in which the KIAA0319L gene has been disrupted using CRISPR/Cas9-mediated gene editing. This polyclonal knockout pool retains genetic diversity while eliminating functional expression of the KIAA0319L protein, enabling robust loss-of-function studies without the clonal artifacts associated with single-cell-derived lines. The product is supplied as a live cell population, ready for expansion and downstream analysis.
HEK293T cells are a widely utilized human embryonic kidney cell line stably expressing the SV40 large T-antigen. This immortalized line is distinguished by its high transfection efficiency, rapid growth, and capacity for high-level protein production, making it a preferred host for viral vector production, signal transduction research, and overexpression studies. Its well-characterized biology and ease of genetic manipulation provide a reliable backdrop for generating knockout models to investigate gene function.
KIAA0319L encodes a multiligand cell surface receptor critically involved in adeno-associated virus (AAV) entry, where it acts as the primary binding receptor for AAV capsid proteins. Beyond its well-established role in viral transduction, KIAA0319L participates in cell-matrix adhesion through cytoskeletal reorganization and has been implicated in neuronal development. Mechanistically, KIAA0319L interacts with AAV capsids and the transmembrane protein GPR108, facilitating clathrin-mediated endocytosis and subsequent endosomal trafficking. Downstream events involve dynamin, Rab proteins, and adaptor complexes, while upstream regulation is governed by neural transcription factors and epigenetic modulators. Its knockout disrupts AAV binding and internalization, abolishing viral transduction and perturbing adhesive functions.
In the HEK293T background, deletion of KIAA0319L generates a cell model that is refractory to AAV infection, making it an invaluable tool for dissecting AAV entry pathways and screening engineered capsids for receptor-independent transduction. Moreover, the knockout provides a clean system to examine KIAA0319L??s contribution to cell adhesion and migration, independent of its viral receptor function. The polyclonal nature avoids biases from clonal selection, and the robust HEK293T platform facilitates high-throughput assays and biochemical analyses that benefit from the cell line’s protein production capacity.
Researchers can employ these polyclonal knockout cells for AAV transduction efficiency assays, where infection rates are compared to wild-type controls using fluorescent or luminescent reporter vectors. Additional applications include co-immunoprecipitation to map KIAA0319L?Ccapsid interactions, immunofluorescence for receptor localization studies, and western blotting to monitor downstream endocytic signaling. The cells are also suitable for adhesion and spreading assays to explore KIAA0319L??s role in cell-matrix interactions. For further information, technical support, or customized solutions, please contact Ascent Research.
