The KIAA1217 Knockout A-549 Polyclonal Cells product comprises a heterogeneous population of A-549 human lung adenocarcinoma cells that have been subjected to CRISPR/Cas9-mediated disruption of the KIAA1217 gene. This polyclonal knockout pool offers a genetically diverse loss-of-function model in which the target gene is ablated across the population without clonal selection, preserving biological variability. The product is supplied as a live cell culture suitable for immediate experimental use.
The parental A-549 cell line is a well-established model of human alveolar epithelial cells, originally derived from a 58-year-old male with lung adenocarcinoma. These cells exhibit epithelial morphology and are widely employed as a model system for studying lung adenocarcinoma biology, including cell adhesion, migration, and signal transduction processes. Their adherent growth and robust experimental tractability make them an ideal host for gene knockout studies.
KIAA1217 encodes a poorly characterized protein that is predicted to participate in actin cytoskeleton organization. Based on bioinformatic and contextual evidence, the KIAA1217 protein likely functions downstream of integrin receptors and Rho family GTPases, including RhoA, Rac1, and Cdc42, which transduce extracellular mechanical cues. It may act as a scaffold or linker, interacting with actin filaments and potentially other cytoskeletal proteins. The mechanistic summary suggests that KIAA1217 knockout is anticipated to alter actin cytoskeleton dynamics, with downstream effects on focal adhesion kinase (FAK) activity and cell migration effectors, thereby impairing cell adhesion and motility.
In the context of A-549 lung adenocarcinoma cells, disruption of KIAA1217 provides a valuable tool to dissect the molecular mechanisms governing tumor cell migration and invasion. The knockout model is expected to exhibit altered cytoskeletal architecture and adhesion dynamics, offering insights into how this uncharacterized protein contributes to the malignant phenotype. Given the importance of actin remodeling in cancer progression, this cell population enables investigation of KIAA1217’s role in cytoskeletal regulation pathways critical for metastasis.
Researchers can exploit this polyclonal knockout population for functional characterization of KIAA1217, including cancer cell migration and invasion studies using wound healing and transwell invasion assays. It is also suitable for protein?Cprotein interaction identification through co-immunoprecipitation and immunofluorescence, as well as for analyzing changes in cell proliferation. Representative assays include western blotting for downstream targets like FAK, RT-qPCR for gene expression profiling, and immunofluorescence to visualize actin cytoskeleton reorganization. For further technical specifications or ordering information, please contact Ascent Research.
