The KIAA1217 Knockout SK-HEP-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population derived from SK-HEP-1, designed for loss-of-function studies of the KIAA1217 gene, which encodes coiled-coil domain-containing protein 57 (CCDC57). This heterogeneous knockout pool allows pooled functional screening while avoiding clonal biases. The model is optimized for investigating cytoskeletal organization, cell motility, and cancer-relevant signaling.
SK-HEP-1, the parental line, is an adherent, endothelial-like human hepatic adenocarcinoma cell line originating from ascites of a liver cancer patient. Known for its metastatic phenotype, it expresses endothelial markers and is a standard model for hepatocellular carcinoma (HCC) research. This background provides a context for examining gene functions related to tumor cell migration, invasion, and epithelial-mesenchymal transition.
KIAA1217/CCDC57 is a putative cytoskeletal organizer with coiled-coil domains that facilitate protein interactions. Potential interacting partners include ??-tubulin, ??-catenin, and focal adhesion kinase (FAK), linking it to Wnt and focal adhesion signaling. Representative pathway components such as RhoA and cofilin suggest that KIAA1217 disruption may impair actin dynamics, cell adhesion, and motility. Although upstream regulators remain unidentified, factors associated with mesenchymal transition are plausible candidates.
In SK-HEP-1 HCC models, KIAA1217 knockout provides a means to dissect the gene??s role in liver cancer progression. The interaction with ??-catenin and FAK situates this protein at a signaling hub frequently altered in HCC, potentially coordinating cytoskeletal rearrangements and adhesion. Moreover, associations with adolescent idiopathic scoliosis and disc degeneration imply broader functions in connective tissue biology, making this model relevant for studying both cancer and musculoskeletal disorders.
This knockout pool is suitable for RT-qPCR, western blotting, immunofluorescence, wound healing, transwell invasion, and RNA-seq experiments. Clonal isolation allows generation of isogenic lines for detailed mechanistic studies. Applications include functional analysis of disease-associated variants, high-throughput drug screening, and exploration of actin regulatory networks. For ordering and technical support, please contact Ascent Research.
