The NHSL3 Knockout HT29 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human colorectal adenocarcinoma cell line HT29, engineered to disrupt the NHSL3 gene. This loss-of-function model enables investigation of NHSL3’s role in actin cytoskeleton organization and cell adhesion. The polyclonal format provides a diverse genetic background, avoiding clonal artifacts while maintaining targeted gene disruption. This product is designed for functional studies in colorectal cancer research.
HT29 cells originate from a primary colorectal adenocarcinoma of a 44-year-old female and exhibit epithelial morphology. Widely used as an intestinal epithelial model, HT29 cells facilitate studies on colorectal cancer biology, intestinal barrier function, and drug responses. Their well-characterized signaling pathways and growth characteristics make them a robust platform for genetic perturbation and phenotypic assays.
NHSL3 belongs to the NHS protein family, which is implicated in actin dynamics and cell adhesion. Although its precise function remains poorly characterized, NHSL3 is predicted to interact with actin and participate in focal adhesion complexes involving proteins such as focal adhesion kinase (FAK) and paxillin. CRISPR-mediated disruption of NHSL3 in HT29 cells may perturb actin filament organization and adhesion signaling, providing a means to examine its contribution to cytoskeletal regulation. Since upstream activators and downstream targets are undefined, this knockout model serves as a critical tool for elucidating NHSL3’s position within the adhesion signaling network.
In the context of HT29 colorectal cancer cells, loss of NHSL3 function may impact epithelial cell behavior, including adhesion, migration, and potentially invasive properties. This model enables researchers to dissect the role of NHSL3 in cancer cell biology, particularly in processes governed by the actin cytoskeleton and focal adhesions. By studying NHSL3-deficient HT29 cells, one can assess alterations in cell morphology, adhesion strength, and wound healing, which are relevant to tumor progression and metastasis.
This polyclonal knockout cell population is suitable for a range of experimental approaches, including cell adhesion assays, scratch wound healing/migration assays, immunofluorescence staining for actin and focal adhesion markers (FAK, paxillin), Western blotting for adhesion proteins, proliferation assays (MTT, colony formation), and drug sensitivity screening. These applications facilitate functional analysis of NHSL3 in colorectal cancer and investigation of actin cytoskeleton regulation. For additional product information and technical support, please contact Ascent Research.
