The KIAA1671 Knockout HT29 Polyclonal Cells consist of a CRISPR/Cas9-edited polyclonal population derived from the HT29 human colorectal adenocarcinoma cell line, featuring targeted disruption of the KIAA1671 gene. This knockout model is generated via CRISPR/Cas9-mediated gene disruption, yielding a heterogeneous pool of cells with diverse loss-of-function mutations. The polyclonal format avoids clonal selection biases, providing a robust resource for functional studies of KIAA1671 in colorectal cancer biology.
HT29 is an established epithelial cell line isolated from a colorectal adenocarcinoma of a 44-year-old female. It is extensively employed as a model for colon cancer, intestinal differentiation, and drug transport studies. HT29 cells recapitulate key features of colorectal malignancy, including dysregulated proliferation and DNA repair. Performing KIAA1671 knockout in this context allows investigation of the gene??s role within a clinically relevant adenocarcinoma background, enhancing translational relevance for colorectal cancer research.
KIAA1671 encodes a poorly characterized protein implicated in cell cycle regulation and genomic stability. It is hypothesized to function within the DNA damage response network, a critical pathway for maintaining genome integrity. Although the upstream regulators, downstream effectors, and interaction partners of KIAA1671 remain unidentified, disruption of this gene in HT29 cells is anticipated to perturb cell cycle progression and DNA repair capacity. This may lead to impaired proliferation and altered cellular responses to genotoxic stress, providing a foundation for mechanistic studies.
Knockout of KIAA1671 in HT29 cells creates a powerful model for dissecting its contribution to colorectal cancer pathogenesis. The loss of a putative genomic stability factor may exacerbate existing defects in DNA damage response pathways inherent to HT29 cells, potentially influencing tumor aggressiveness and therapeutic sensitivity. This model enables comparative analysis of proliferation, apoptosis, migration, and drug response between knockout and parental cells, shedding light on KIAA1671-dependent phenotypes in colorectal adenocarcinoma.
These polyclonal knockout cells are amenable to a wide range of assays, including western blotting, RT-qPCR, immunofluorescence, flow cytometry, and functional evaluations such as apoptosis assays, migration/invasion studies, cell cycle analysis, and drug sensitivity testing. They support high-throughput screening of anticancer compounds and functional genomics applications aimed at validating KIAA1671 as a potential target. For additional information on this product or technical assistance, please contact Ascent Research.
