The KIAA1671 Knockout Jurkat Polyclonal Cells product provides a CRISPR/Cas9-edited polyclonal knockout cell population in the human Jurkat T-lymphocyte line, designed to disrupt the KIAA1671 gene. This polyclonal format comprises a heterogeneous pool of cells harboring diverse Cas9-mediated target-gene disruptions, minimizing clonal bias and enabling robust functional studies. The product is supplied as a ready-to-use, proliferating cell population ideal for downstream phenotypic assays, functional characterization, and comparative studies with wild-type Jurkat cells.
Jurkat cells, derived from the peripheral blood of a 14-year-old male with acute T-cell leukemia, are a widely established model for T-cell acute lymphoblastic leukemia (T-ALL) and T-cell receptor (TCR) signaling studies. These suspension cells express typical T-lymphocyte markers and exhibit antigen-independent constitutive signaling characteristics that make them instrumental for dissecting TCR-proximal events, leukemia biology, and immune cell activation mechanisms. Their rapid growth and history of genetic characterization further support reproducible loss-of-function investigations.
KIAA1671 is an uncharacterized protein featuring a domain of unknown function (DUF4688) and is predicted to participate in cytoskeletal dynamics. No upstream regulators, downstream targets, or interacting partners have been identified, and its mechanistic role remains completely undefined. Consequently, targeted disruption of KIAA1671 in a well-characterized T-cell background provides a clean, unbiased platform to probe its potential involvement in processes such as actin remodeling, cell migration, or intracellular trafficking, without preconceived pathway assignments.
Within the Jurkat host context, KIAA1671 knockout cells enable the dissection of this orphan gene??s contribution to T-lymphocyte biology and leukemia-relevant phenotypes. Techniques such as proliferation assays, flow cytometry, immunofluorescence, and migration assays can be employed to screen for alterations in cell cycle progression, surface marker expression, cytoskeletal organization, or chemotactic responses. The polyclonal design ensures that observed phenotypes are not artifacts of a singular clone, strengthening the biological conclusions drawn from experimental data.
This knockout model is ideally suited for functional elucidation of KIAA1671, as well as studies in T-cell biology, leukemia research, and cytoskeletal dynamics. Researchers can utilize Western blotting and RT-qPCR to confirm gene disruption at the protein and transcript levels, viability assays to assess cellular fitness, and immunofluorescence to visualize subcellular structures. The cells are compatible with a broad range of standard mammalian cell culture conditions and genetic manipulation workflows. For additional details or ordering information, contact Ascent Research.
