KIAA1671 Knockout NCI-H1975 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population of NCI-H1975 human lung adenocarcinoma cells with disruption of the KIAA1671 gene, enabling loss-of-function phenotypic analyses without clonal artifacts. This heterogeneous knockout model provides a resource for studying the role of the uncharacterized KIAA1671 protein in non-small cell lung cancer (NSCLC).
NCI-H1975 is an epithelial cell line derived from the pleural effusion of a non-smoking female patient with lung adenocarcinoma and are derived from a metastatic site, thus supporting invasion studies. It harbors EGFR L858R/T790M and PIK3CA mutations, conferring resistance to first-generation EGFR inhibitors and constitutively active PI3K/AKT signaling. This genotype makes the cell line a widely used model for studying resistance mechanisms and therapeutic interventions in NSCLC.
The KIAA1671 protein is predicted to regulate cell proliferation and cytoskeletal dynamics, though its molecular function remains to be defined. It may be controlled by EGF/EGFR signaling and is thought to interact with cell cycle regulators Cyclin D1 and CDK4/6, as well as actin and tubulin, placing it at a nexus of growth factor and cytoskeletal signaling. In NCI-H1975 cells, KIAA1671 disruption is anticipated to impair EGFR-driven signaling through MAPK1/2 and AKT, leading to reduced proliferative and migratory capacity.
Within the NCI-H1975 background, characterized by EGFR T790M and PIK3CA mutations, loss of KIAA1671 may selectively attenuate oncogenic signaling pathways. By assessing mutant EGFR and PI3K signaling nodes, researchers can delineate genotype-specific vulnerabilities. The knockout model enables investigation of how this uncharacterized gene contributes to proliferation, colony formation, and migration under constitutively active PI3K/AKT and MAPK conditions, providing a relevant platform to uncover functional dependencies in lung adenocarcinoma cells resistant to targeted therapies.
This polyclonal knockout population is well suited for a range of functional assays, including cell proliferation (MTT/CCK-8) to gauge growth rates, Western blotting for protein expression analysis, migration (wound healing/transwell) assays to evaluate motility, colony formation for clonogenic potential, flow cytometry for cell cycle distribution, and RT-qPCR for transcript quantification. Applications encompass gene function studies, drug target discovery, and cancer cell signaling research. For further technical details, please contact Ascent Research.
