The KTN1 Knockout NCI-H1975 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting the KTN1 gene in human NCI-H1975 non-small cell lung cancer (NSCLC) cells. This product consists of a heterogeneous pool of cells with CRISPR/Cas9-mediated gene disruption, eliminating kinectin protein expression without clonal uniformity. It serves as a defined model to study kinectin-dependent endoplasmic reticulum (ER) functions and microtubule interactions in lung adenocarcinoma.
The host NCI-H1975 cell line is an epithelial lung adenocarcinoma model derived from a female NSCLC patient, carrying an activating EGFR L858R mutation and a T790M resistance mutation. These genetic features make it a clinically relevant system for studying EGFR tyrosine kinase inhibitor (TKI) resistance, tumor progression, and metastatic behavior. The line exhibits invasive potential and is widely employed in migration and drug sensitivity assays.
KTN1 encodes kinectin, a coiled-coil ER membrane protein that anchors kinesin-1 (KIF5B) to mediate microtubule-dependent ER tubulation, vesicle transport, and organelle positioning. Kinectin integrates upstream signals from EGFR and calpain proteolysis, and its downstream effects include regulation of integrin ??1-mediated focal adhesion assembly and microtubule stability. It interacts with tubulin and Rho GTPases, connecting ER dynamics to cytoskeletal reorganization and integrin signaling. Within the EGFR-mutant context, KTN1 sits at a hub linking growth factor pathways (PI3K/AKT/mTOR) to cell-matrix adhesion.
In NCI-H1975 cells, KTN1 disruption is expected to impair ER network organization and kinesin-driven trafficking, potentially attenuating integrin-based signaling and downstream PI3K/AKT activity. This modulation may alter cell migration, invasion, and responses to EGFR inhibitors such as osimertinib, providing a tractable model to examine kinectin??s role in drug resistance and metastatic phenotypes. The polyclonal knockout format mitigates clonal selection artifacts, preserving heterogeneous responses relevant to tumor biology.
These polyclonal knockout cells are suitable for Western blot analysis of KTN1 and phospho-AKT, immunofluorescence visualization of ER and microtubules, co-immunoprecipitation of KIF5B, and functional assays like migration/invasion and osimertinib sensitivity testing. They enable investigations into ER stress?Cintegrin crosstalk, synthetic lethality screening in EGFR-mutant NSCLC, and ER-microtubule dynamics in metastasis. For additional information or customized applications, contact Ascent Research.
