LHFPL2 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from the Raji B lymphocyte line. This model offers loss-of-function for studying LHFPL2, a tetraspan membrane protein involved in BMP and Wnt signaling. The polyclonal format provides a genetically diverse knockout pool without clonal selection, enabling robust functional studies in a suspension lymphoblastoid background.
The Raji host cell line, originating from human Burkitt lymphoma and EBV-positive, serves as a widely used model for B cell biology. It grows in suspension and exhibits rapid proliferation, facilitating assays in signal transduction and cancer biology. The EBV status and lymphoblastoid features offer a relevant context for exploring tetraspanin-mediated signaling in B cell malignancy.
LHFPL2 negatively regulates BMP signaling by binding BMPR-II and inhibiting SMAD1/5/8 phosphorylation, thereby reducing transcription of targets like ID1 and ID2. It also organizes tetraspanin-enriched microdomains, interacting with CD9, CD81, and integrins to modulate Wnt signaling and cell adhesion. Upstream regulators include BMP ligands and SOX2; downstream, it influences Wnt components such as FZD receptors, LRP5/6, and beta-catenin. This positions LHFPL2 at a signaling nexus coordinating BMP and Wnt pathways.
In Raji cells, LHFPL2 knockout enables dissection of BMP/Wnt crosstalk in B cell adhesion and lymphoma biology. The model is pertinent for investigating tetraspanin-mediated membrane organization in malignant B cells. Its polyclonal nature avoids artifacts, reflecting population-level effects crucial for heterogeneous lymphoma studies. Research links LHFPL2 to hearing loss, lipoma, and glioma, underscoring broader biological significance.
These cells support applications such as Western blotting for phospho-SMAD1/5, RT-qPCR for ID1/ID2, and co-immunoprecipitation of LHFPL2-BMPR-II complexes. Flow cytometry monitors surface integrin changes; immunofluorescence reveals tetraspanin network alterations. Transcriptomic analyses like RNA-seq identify global expression shifts due to LHFPL2 disruption. These approaches facilitate mechanistic studies of BMP/Wnt integration in B lymphocytes and screening for lymphoma targets. For technical inquiries, contact Ascent Research.
