The LMTK2 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji human B lymphocyte line. This heterogeneous pool of cells carries targeted disruption of the LMTK2 gene, creating a versatile loss-of-function model. As a polyclonal population, it captures genetic diversity inherent to bulk gene editing, making it suitable for assays where population-level effects are of primary interest.
The parental Raji cell line is an EBV-positive Burkitt lymphoma B lymphocyte model extensively utilized in immunological and cancer research. Raji cells maintain robust antibody production and efficient antigen presentation, serving as a preferred platform for studying B cell receptor signaling, immune synapse formation, and endocytic processes. Their well-characterized genetic background and culture tractability make them ideal for functional genomics and drug discovery.
LMTK2 encodes a serine/threonine kinase that coordinates endocytic trafficking by phosphorylating myosin VI. Activation occurs downstream of TGF-beta and growth factor receptors, linking extracellular signals to clathrin-mediated endocytosis. LMTK2 directly interacts with DAB2 and the AP-2 clathrin adaptor complex at the plasma membrane to mediate internalization of cargoes such as the transferrin receptor. By coupling receptor activation to myosin VI-driven vesicle scission, LMTK2 serves as a central regulator of endocytic flux and has broader roles in apoptosis and neurite outgrowth.
In Raji B lymphocytes, disruption of LMTK2 significantly impairs clathrin-mediated endocytosis and myosin VI-dependent trafficking, which are critical for antigen internalization and surface receptor homeostasis. Aberrant endocytic flux may result in altered expression of cargo receptors such as the transferrin receptor and perturbed downstream TGF-beta and growth factor signaling. Defective trafficking also impacts apoptotic signaling and cytokine receptor dynamics, offering a model to investigate the crosstalk between membrane trafficking and cell death in B cell malignancies. The polyclonal knockout population captures diverse mutational outcomes, providing a robust tool for analyzing population-level endocytic phenotypes.
This LMTK2 knockout polyclonal cell product supports a broad array of applications, including detailed mechanistic studies of endocytic trafficking, TGF-beta signaling, and apoptosis regulation in B cell contexts. It is well-suited for cancer research exploring Burkitt lymphoma and prostate cancer pathways where LMTK2 is implicated. Researchers can utilize western blotting to confirm LMTK2 ablation, immunofluorescence for transferrin uptake, co-immunoprecipitation to probe interactions with myosin VI and AP-2, and flow cytometry for endocytic markers. Apoptosis assays additionally enable functional dissection of cell death pathways. For pricing, availability, or technical inquiries, please contact Ascent Research.
