The LPP Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed for targeted disruption of the LPP gene in human Raji B lymphoblastoid cells. This heterogeneous pool enables robust loss-of-function studies without the constraints of clonal isolation, preserving inherent biological variability. The model serves as a versatile platform for investigating LPP??s dual roles as a scaffold protein and transcriptional coactivator in focal adhesion dynamics, cell migration, and proliferative signaling.
Raji is an EBV-positive Burkitt??s lymphoma-derived B lymphoblastoid cell line extensively used in immune response and lymphoma research. It exhibits surface immunoglobulin markers characteristic of mature B cells and is amenable to genetic manipulation, making it a well-established system for studying B-cell receptor signaling, antibody production, and oncogenic transformation. Its suspension growth and transfection efficiency facilitate functional genomic analyses.
LPP localizes to focal adhesions and cell?Ccell junctions, where it links the actin cytoskeleton to signaling complexes through interactions with ??-actinin, zyxin, and vinculin. Upon stimulation by upstream regulators such as TGF-??, integrin ligation, mechanical stress, or RhoA activation, LPP translocates to the nucleus and acts as a coactivator for SRF via MRTF-A/MKL1 and for ??-catenin-dependent transcription through TCF/LEF, in part by cooperating with Smad3. This dual cytoplasmic/nuclear function integrates mechanical and biochemical cues to drive gene programs associated with epithelial?Cmesenchymal transition (EMT), proliferation, and migration.
In the Raji background, LPP knockout allows dissection of how loss of focal adhesion integrity and transcriptional coactivation influences lymphoblastoid cell behavior. Given LPP??s links to cancer metastasis, acute myeloid leukemia, and invasive ductal carcinoma, this model may reveal alterations in adhesion, migration, and TGF-?? responsiveness relevant to lymphoma dissemination and other hematologic malignancies.
Researchers can employ these cells in Western blotting for LPP and downstream effectors, immunofluorescence for focal adhesion components, transwell migration and invasion assays, luciferase reporter assays for SRF or TCF/LEF activity, co-immunoprecipitation to map interaction networks, RNA-seq for transcriptomic profiling, and phospho-Smad analysis. Applications span focal adhesion biology, TGF-??-induced EMT, cell invasion mechanisms, transcriptional regulation, and drug screening for anti-invasive agents. For further information or technical support, please contact Ascent Research.
