The LRP1 Knockout IPEC-J2 Cell Line is a CRISPR/Cas9-edited knockout cell line featuring targeted disruption of the LRP1 gene in the porcine intestinal epithelial cell line IPEC-J2. LRP1 encodes the low-density lipoprotein receptor-related protein 1, a multifunctional endocytic receptor mediating ligand internalization and signal transduction. This loss-of-function model provides a powerful tool for studying LRP1-dependent processes in an intestinal epithelial context.
The IPEC-J2 cell line is a non-transformed continuous line derived from the jejunum of a neonatal piglet. These cells form polarized monolayers with tight junctions and express characteristic intestinal epithelial markers, including microvilli, nutrient transporters, and junctional proteins. They exhibit high transepithelial electrical resistance (TEER) and are extensively used to model intestinal barrier function, nutrient absorption, host-microbe interactions, and drug transport.
LRP1 functions as a scavenger receptor that binds a broad array of ligands such as ApoE, ??2-macroglobulin, tPA, and PAI-1, facilitating their clathrin-mediated endocytosis and lysosomal degradation. This endocytic activity regulates lipoprotein metabolism and proteinase clearance. Beyond endocytosis, LRP1 couples to intracellular signaling networks; it interacts with adaptors like Dab1 and PSD-95, and cooperates with integrins and PDGFR?? to control cell adhesion and migration. Downstream signaling through PI3K/Akt and MAPK/ERK pathways modulates ERK1/2, Akt, JNK, NF-??B, and MMPs, thereby influencing proliferation, survival, and motility.
Disruption of LRP1 in IPEC-J2 cells is projected to impair ligand clearance and perturb signaling cascades essential for epithelial homeostasis. Given LRP1??s roles in adhesion and migration, knockout cells may exhibit compromised barrier integrity and altered immune surveillance. This model enables investigation of LRP1-mediated mechanisms in a non-transformed intestinal epithelium, offering insights into how endocytic defects contribute to gut pathophysiology, including inflammatory and metabolic disorders.
The knockout cell line is suited for diverse functional assays such as endocytosis uptake assays, transwell migration assays, western blotting for signaling proteins (e.g., phospho-ERK1/2, Akt), immunofluorescence, and TEER measurements. Research applications include studies on intestinal epithelial biology, cholesterol trafficking, host-pathogen interactions, barrier integrity, and drug delivery. It also facilitates exploration of LRP1-related diseases like Alzheimer??s disease, atherosclerosis, and cancer metastasis. For additional information, please contact Ascent Research.
