MCTP2 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human Raji B lymphocyte cell line, featuring targeted disruption of the MCTP2 (Multiple C2 and Transmembrane Domain Protein 2) gene. This heterogeneous polyclonal pool comprises a mixture of cells carrying diverse CRISPR/Cas9-mediated edits at the MCTP2 locus, providing a loss-of-function model system for investigating MCTP2-dependent biological processes without the clonal selection bottleneck.
The host Raji cell line originates from a Burkitt??s lymphoma patient and maintains an Epstein-Barr virus (EBV)-positive, B-lymphocyte identity. Raji cells exhibit hallmark features of antigen-presenting B cells, including robust immunoglobulin secretion, surface expression of MHC class II molecules, and the capacity to engage in B cell receptor (BCR)-mediated signaling. Their transformed yet functionally competent phenotype makes them a widely used model for dissecting B-cell malignancies, immune synapse formation, and lymphocyte exocytosis.
MCTP2 functions as a calcium-sensing protein that bridges intracellular calcium signals to SNARE-mediated membrane fusion events. Upon increases in cytosolic calcium??triggered by BCR stimulation or store-operated calcium entry??MCTP2 is thought to interact with core SNARE complex components such as syntaxin, SNAP-25, and VAMP, alongside regulatory cofactors like synaptotagmin and complexin, to promote vesicle docking and exocytosis. Consequently, MCTP2 operates at the nexus of calcium signaling and SNARE assembly, with downstream consequences for secretory vesicle fusion and release of preformed cargo.
In the Raji B-cell context, disruption of MCTP2 is predicted to impair calcium-dependent exocytic pathways, potentially attenuating secretion of cytokines, antibodies, or other effector molecules critical for immune function. This model may uncover novel roles for MCTP2 in B-cell antigen presentation, cytokine-mediated communication, and the maintenance of oncogenic signaling networks in Burkitt??s lymphoma. The polyclonal nature of the knockout population allows researchers to assess phenotypic heterogeneity and avoid artifacts linked to single-cell clonal expansion.
These MCTP2 knockout Raji polyclonal cells are ideally suited for mechanistic studies of SNARE regulation in lymphocytes, high-throughput screening of small molecules that modulate B-cell secretion, and comparative proteomic analyses of calcium-dependent trafficking machinery. Representative assays include Western blotting to confirm MCTP2 ablation, RT-qPCR for transcript-level validation, flow cytometry for surface marker profiling, cytokine secretion ELISA, calcium flux assays using fluorescent indicators, co-immunoprecipitation of SNARE complexes, and apoptosis assays to evaluate survival effects. For additional product specifications, please contact Ascent Research.
