The MID1IP1 Knockout Raji Polyclonal Cells comprise a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji human B lymphocyte line, with disruption of the MID1IP1 gene. Targeted gene disruption was achieved using CRISPR/Cas9 technology, yielding a heterogeneous pool suitable for bulk loss-of-function assays while minimizing clonal selection artifacts.
The Raji host cell line is a suspension-adapted, Epstein-Barr virus (EBV)-positive lymphoblastoid line established from a Burkitt lymphoma patient. This model recapitulates key aspects of B-cell malignancy, including deregulated proliferation and active metabolic pathways. Well-characterized transcriptomic and proteomic profiles of Raji cells enhance the interpretability of functional genomics studies focused on cancer metabolism.
MID1IP1 (also known as MIG12) encodes a pivotal regulator of de novo lipogenesis. Mechanistically, MID1IP1 partners with Spot14 (THRSP) to stimulate acetyl-CoA carboxylase alpha (ACC), the rate-limiting enzyme for malonyl-CoA production. This drives downstream expression of fatty acid synthase (FASN) and stearoyl-CoA desaturase 1 (SCD1), and promotes lipid droplet accumulation via perilipins PLIN2 and PLIN3. MID1IP1 transcription is governed by SREBP-1c and ChREBP, activated by insulin and LXR signaling. Additionally, MID1IP1 interacts with MID1 and intersects with AMPK and mTOR pathways, linking nutrient sensing to lipid anabolism.
In Raji lymphoma cells, de novo lipogenesis supports membrane biogenesis and signaling lipid pools. Disruption of MID1IP1 impairs ACC activation, reducing malonyl-CoA, fatty acid synthesis, and lipid storage. The EBV-driven metabolic reprogramming in Raji cells suggests MID1IP1 knockout may expose viral-associated vulnerabilities, offering a model to study oncogenic and metabolic pathway intersections.
Researchers can employ this model for diverse downstream analyses. Routine validation includes Western blotting and RT-qPCR for MID1IP1 and lipogenic targets. Lipid droplets can be visualized with BODIPY or Oil Red O, and ACC activity assessed enzymatically. Cell proliferation, cell cycle, and apoptosis are measured by MTT, flow cytometry, and related methods; RNA-seq enables transcriptome-wide analysis. These cells are suited for anti-lipogenic target validation and functional genomics. For further information, please contact Ascent Research.
