The MMRN1 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji human B lymphocyte cell line. This product enables loss-of-function studies of the MMRN1 gene, which encodes multimerin-1, a soluble adhesive protein. The knockout was generated through CRISPR/Cas9-mediated gene disruption, yielding a heterogeneous population of cells with targeted MMRN1 ablation. This polyclonal format is well-suited for applications requiring a pool of knockout cells, such as functional genomics screens and negative controls for antibody validation.
The host cell line, Raji, is a suspension lymphoblast cell line established from a patient with Burkitt’s lymphoma. These B lymphocytes are EBV-positive and display characteristics of a mature B cell phenotype. Raji cells are widely used in biomedical research for studying B cell biology, oncogenic mechanisms, and the role of Epstein-Barr virus in lymphomagenesis. Their ease of culture and extensive characterization make them a robust platform for genetic manipulation and downstream functional assays.
MMRN1 is a soluble adhesive protein stored in platelet ??-granules and endothelial cells. Upon activation by thrombin, collagen, ADP, and VEGF, it is released and participates in hemostasis and angiogenesis. MMRN1 carries coagulation factor V, stabilizing it and enhancing its procoagulant function, and binds integrins ??IIb??3 and ??v??3, promoting platelet adhesion, aggregation, and endothelial cell adhesion. Downstream effects include fibrin clot formation and angiogenic signaling via VEGFR2, involving interactions with factor Va, factor XIIIa, fibrinogen, and fibronectin.
Although MMRN1 expression is not typical of lymphoid cells, ectopic expression or induction may influence B cell adhesion and integrin signaling. The MMRN1 knockout in Raji cells provides a model to dissect non-hemostatic roles of MMRN1 in lymphocyte biology and B cell lymphoma pathogenesis, enabling investigation of integrin activation profiles, cell adhesion, and migration.
These cells support assays such as Western blotting, RT-qPCR, and Sanger sequencing for knockout confirmation. Functional assays include cell adhesion and migration, flow cytometry for integrin expression, and co-immunoprecipitation to assess factor V binding. They serve as a negative control for MMRN1 antibody validation and can be used in platelet aggregation assays after differentiation into platelet-like cells. The MMRN1 Knockout Raji Polyclonal Cells are a versatile tool for hemostasis research, cancer biology, and functional genomics. For further information, contact Ascent Research.
