The MPP7 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population derived from Raji B lymphocytes, featuring targeted disruption of the MPP7 gene. This heterogeneous pool enables loss-of-function studies without clonal selection, suitable for bulk analyses where diverse editing events are acceptable. The knockout model provides a versatile system for investigating MPP7 scaffold protein function in a lymphoid background.
The Raji cell line is a human Burkitt??s lymphoma-derived B lymphocyte line that is Epstein-Barr virus (EBV)-positive and widely used in immunology and cancer research. Raji cells grow in suspension, express B-cell markers, and are capable of antibody production and antigen presentation, making them a relevant host for studying lymphocyte biology, immune signaling, and lymphomagenesis in the context of MPP7 disruption.
MPP7 is a membrane-associated guanylate kinase (MAGUK) scaffold protein essential for tight junction assembly and apicobasal polarity. It functions by anchoring the DLG1?CLIN7 complex to the plasma membrane, where it interacts with tight junction proteins including TJP1 (ZO-1), OCLN, CLDN, and F11R (JAM-A). Upstream regulators such as Wnt signaling, p63, and cell density modulate MPP7 activity. The scaffold also associates with INADL and MPDZ, integrating signals that stabilize junctional complexes. MPP7 loss disrupts tight junction integrity, which can lead to loss of polarity and EMT-like changes. Pathway partners frequently studied alongside MPP7 include TJP2, PARD3, and PRKCZ.
Although Raji cells are lymphoid and lack typical tight junctions, they express several junction-associated proteins, enabling investigation of non-canonical MPP7 roles in B cells. The knockout model aids in studying MPP7??s impact on lymphocyte adhesion, migration, and immune synapse organization, processes relevant to antigen presentation and lymphoma progression. Moreover, MPP7??s interaction with DLG1, a protein implicated in immune cell signaling and tumor suppression, makes the Raji background valuable for dissecting these complexes in a hematopoietic malignancy setting.
Researchers can use these cells for co-immunoprecipitation to identify MPP7 interaction partners, RT-qPCR and Western blotting to assess gene and protein expression, and immunofluorescence microscopy to examine protein localization. Flow cytometry facilitates analysis of surface markers or cell cycle effects. Functional assays such as transwell migration or adhesion tests may reveal MPP7-dependent phenotypes in B cells. The polyclonal format supports pooled screens and inhibitor validation. For further details, pricing, or bulk inquiries, contact Ascent Research.
