MPV17 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the MPV17 gene. MPV17 encodes a mitochondrial inner membrane protein critical for mtDNA maintenance, and its disruption provides a loss-of-function model for studying mitochondrial nucleotide homeostasis and respiratory chain function. The polyclonal format ensures a heterogeneous editing profile, enabling robust assessment of MPV17 deficiency without clonal bias.
Raji is a human B-lymphocyte cell line derived from a Burkitt lymphoma and transformed by Epstein?CBarr virus (EBV). These cells express characteristic B-cell markers such as CD19 and CD20, and are widely employed in immunology and cancer research due to their rapid proliferation, ease of genetic manipulation, and relevance to B-cell malignancies.
MPV17 functions as a non?selective channel or nucleotide transporter in the mitochondrial inner membrane, supplying deoxynucleotide triphosphates (dNTPs) essential for mtDNA replication. Its expression is transcriptionally regulated by the mitochondrial biogenesis factors PGC?1?? (PPARGC1A), NRF1, and TFAM. Disruption of MPV17 impairs mitochondrial nucleotide pools, leading to reduced mtDNA copy number, loss of mitochondrial membrane potential, diminished ATP synthesis, and elevated reactive oxygen species (ROS). Consequently, the activity of oxidative phosphorylation (OXPHOS) complexes I?CV is compromised, and cellular energy metabolism shifts.
In the Raji B?cell background, MPV17 knockout provides a relevant model to investigate how mitochondrial dysfunction intersects with B?cell lymphoma biology. The model can be used to examine the consequences of mtDNA instability on proliferation, survival, apoptotic signaling, and metabolic reprogramming in malignant B cells, potentially informing our understanding of treatment resistance mechanisms.
Key research applications include modeling mitochondrial DNA depletion syndromes, performing functional studies via mtDNA quantitative PCR, Seahorse respirometry, ATP level quantification, mitochondrial membrane potential assays (TMRE or JC?1), ROS detection, western blotting for OXPHOS subunits, and annexin V apoptosis assays. This product also facilitates drug screening for mitochondrial diseases. For additional technical information, ordering details, or custom gene?editing services, please contact Ascent Research.
