The MSLN Knockout Raji Polyclonal Cells consist of a CRISPR/Cas9-mediated gene-disrupted pool of Raji B lymphocytes that eliminates mesothelin protein expression. This polyclonal knockout population bypasses clonal selection, offering a heterogeneous loss-of-function model for studying mesothelin??s roles in adhesion, signaling, and disease. By disrupting MSLN, researchers can directly assess the consequences of mesothelin ablation in a well-characterized B lymphoid background.
The Raji host line is an EBV-positive Burkitt lymphoma B cell line expressing CD19, CD20, and CD22. It retains antibody-producing features and is a standard model for B cell biology, lymphomagenesis, and immunological studies. Its rapid growth and genetic manipulability facilitate CRISPR-based knockout generation.
Mesothelin is a GPI-anchored glycoprotein that fosters cell adhesion and tumor progression primarily through interaction with MUC16. It activates NF-??B via IKK-mediated I??B?? degradation and p65 translocation, stimulates the PI3K/AKT/mTOR pathway, and engages the MAPK/ERK cascade (RAS?CRAF?CMEK?CERK), resulting in ERK1/2 and AKT phosphorylation and ??-catenin stabilization. The MSLN gene is regulated by NF-??B, AP-1, Wnt/??-catenin, and TNF-??, and mesothelin cooperates with integrins and galectin-3 to modulate adhesive and survival signals.
Within the Raji B lymphocyte system, MSLN knockout provides a clean background for examining mesothelin-driven signaling independent of native expression. This model is useful for ectopic expression studies and for dissecting how mesothelin influences NF-??B and PI3K/AKT cascades that are central to B cell survival and lymphomagenesis, offering insights into tumor microenvironment and immunotherapy applications.
These knockout cells support cancer research, tumor microenvironment analysis, and immunotherapy target validation. Representative assays include Western blotting and RT-qPCR for MSLN expression, flow cytometry for surface mesothelin, and functional readouts such as phospho-ERK/AKT analysis, proliferation, apoptosis, and NF-??B reporter assays. Co-immunoprecipitation and MUC16 binding studies allow exploration of protein interactions. For further details, please contact Ascent Research.
