The NCEH1 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population for NCEH1 loss-of-function studies. Generated by gene disruption in the Raji cell line, this heterogeneous pool avoids clonal selection artifacts and preserves genetic diversity, providing a robust model for metabolic and oncogenic research.
The Raji cell line is a human B lymphocyte-derived model from Burkitt??s lymphoma, characterized by EBV positivity and a t(8;14) MYC translocation. It grows in suspension and expresses B cell markers, making it a staple in immunology and hematological malignancy studies. This well-characterized lymphoblastoid background is ideal for investigating cholesterol metabolism in lymphoma.
NCEH1 localizes to lipid droplets and hydrolyzes cholesterol esters into free cholesterol and free fatty acids, a key step in cholesterol mobilization. Its expression and activity are controlled by SREBP2, LXR, PPAR??, and cAMP/PKA signaling. NCEH1 cooperates with HSL, ATGL, and perilipins such as PLIN2 and PLIN3, as well as ABHD5. Liberated free cholesterol activates LXR/RXR, inducing ABCA1-mediated efflux, while excess is re-esterified by ACAT. Thus, NCEH1 integrates lipolytic and cholesterol-sensing pathways, influencing lipid raft composition and downstream signaling.
In Raji cells, NCEH1 disruption likely impairs cholesterol ester hydrolysis, altering free cholesterol availability and lipid raft integrity. Since B lymphocytes rely on membrane cholesterol for signaling platforms, this knockout may perturb B cell receptor signaling, survival, and proliferation. The MYC-driven Raji model is sensitive to metabolic changes, making it valuable for exploring cholesterol homeostasis?Concogenic signaling intersections and potential therapeutic vulnerabilities in lymphoma.
This polyclonal knockout cell population enables diverse functional studies. Lipid droplet visualization by BODIPY or Oil Red O staining, cholesterol ester hydrolysis activity assays, and cholesterol efflux measurements can directly probe NCEH1 function. Lipidomics via mass spectrometry allows comprehensive lipid profiling, while Western blotting confirms target disruption. Apoptosis, proliferation, and flow cytometry for lipid raft markers facilitate assessment of NCEH1-dependent phenotypes in lymphoma biology. Applications include cancer cell lipid metabolism, drug resistance, and B cell signaling research. Please contact Ascent Research for further information.
