The NFIC Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population generated from the Raji B lymphocyte line by disrupting the NFIC gene, abrogating its transcriptional activity. This heterogeneous pool serves as a loss-of-function model to study NFIC-dependent gene regulation and signaling in a B cell lymphoma context.
Raji is a human B lymphoblastoid cell line derived from a Burkitt lymphoma patient, characterized by an Epstein?Barr virus (EBV)?positive, mature B cell phenotype. It is extensively employed as a model system for B cell lymphoma research, including studies of lymphomagenesis, B cell receptor signaling, and oncogenic processes. The line??s robust growth and genetic manipulability make it particularly suitable for CRISPR/Cas9-mediated gene disruption.
NFIC, a nuclear factor I family transcription factor, regulates cell proliferation, differentiation, and gene expression through sequence?specific DNA binding. It is activated downstream of TGFB1, BMP2, and WNT3A, and forms regulatory complexes with NFIA, NFIB, NFIX, SP1, FOS, JUN, and TAF4. NFIC directly controls targets such as DSPP, COL1A1, BGLAP, SPP1, FN1, and CDH2, linking TGF???, Wnt, and BMP pathways. Knockout?mediated disruption abolishes its DNA binding and transcriptional activation, potentially altering downstream gene programs in Raji cells with consequences for lymphoma?relevant processes like proliferation and survival.
In the Raji lymphoma background, NFIC loss provides a model to dissect its role in B cell malignancies. NFIC has been associated with lymphoma, glioblastoma, and breast cancer, suggesting broad oncogenic relevance. The knockout allows examination of changes in cell cycle progression, apoptosis, and drug responsiveness, as well as interrogation of NFIC-dependent transcriptional networks that sustain the transformed state.
Applications include drug target validation through proliferation and apoptosis assays, global expression profiling via RNA?seq, chromatin occupancy mapping by ChIP?seq, and protein/mRNA analysis with Western blotting and RT?qPCR. Flow cytometry enables phenotypic characterization. These tools facilitate investigation of transcription factor function in lymphocyte biology, oncogenic signaling, and target gene screening. For ordering or technical inquiries, contact Ascent Research.
