The NQO2 Knockout Raji Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal cell population derived from the Raji B lymphocyte cell line, engineered for targeted disruption of the NQO2 gene. This loss-of-function model is designed to abolish NQO2 expression, providing a heterogeneous knockout background suitable for population-based studies of quinone metabolism and redox signaling without the constraints of clonal selection.
Raji cells are a widely used human B lymphoblastoid line isolated from an EBV-positive Burkitt’s lymphoma patient. They express surface IgM and characteristic B-cell markers, exhibiting constitutive NF-??B activity. The transformed, immortalized nature of Raji cells makes them an ideal host for examining oncogenic pathways and drug responses in B-cell malignancies.
NQO2 (NRH:quinone oxidoreductase 2) catalyzes the two-electron reduction of quinones and aromatic nitro compounds using dihydronicotinamide riboside (NRH) as an electron donor. Its enzymatic activity is regulated by upstream factors including p53, resveratrol, and the aryl hydrocarbon receptor (AhR). NQO2 interacts with resveratrol-binding proteins, amyloid-beta peptide, and proteasome subunits, and its redox output modulates downstream targets such as reactive oxygen species (ROS) generation and nitric oxide synthase (NOS). These interactions place NQO2 at the intersection of xenobiotic detoxification and redox-sensitive NF-??B signaling.
Knockout of NQO2 in Raji cells impairs quinone detoxification, potentially increasing sensitivity to oxidative stress and chemotherapeutic agents. By altering the cellular redox balance, the model may influence NF-??B-mediated survival and inflammatory pathways critical in Burkitt’s lymphoma pathogenesis. This disruption is relevant for studying drug resistance mechanisms and for modeling aspects of NQO2-associated diseases such as Parkinson??s disease.
The NQO2 Knockout Raji Polyclonal Cells are suitable for diverse research applications, including cancer biology, drug resistance studies, redox signaling, and toxicology. Typical assays include western blotting and RT-qPCR for NQO2 expression, quinone reductase activity measurements, cell viability testing with resveratrol or quinone compounds, NF-??B reporter assays, ROS detection, and apoptosis analysis. These cells offer a robust platform for investigating NQO2-related pathways and screening modulators of quinone metabolism. For additional information or customized services, please contact Ascent Research.
