The ODF2 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting the ODF2 gene in Homo sapiens Raji B lymphocytes. This heterogeneous pool provides a loss-of-function model without clonal selection, enabling population-level studies of ODF2??s roles in centrosome biology and ciliogenesis. The product is designed for advanced research applications requiring gene disruption in a relevant cellular context.
The Raji host cell line, derived from Burkitt lymphoma, is EBV-positive, grows in suspension, and exhibits lymphoblastoid morphology. As a classical B lymphocyte model, Raji cells are extensively used for investigating B-cell malignancies, immune signaling, and oncogenic processes. Their robust suspension culture and well-characterized genome make them a reliable platform for CRISPR-edited knockout populations.
ODF2 encodes a centriolar satellite protein critical for primary cilium formation, centrosome duplication, and microtubule organization. The protein interacts with key centrosomal components including Ninein, CEP164, pericentrin, CDK5RAP2, and CEP290. Its expression is regulated by transcription factors FOXJ1, RFX2, and RFX3, and it integrates with cell cycle regulators such as cyclins and CDKs. Downstream, ODF2 influences centrosome cohesion through Ninein and CEP164, while pathway effectors Aurora A kinase, PLK4, SAS-6, and CPAP coordinate ciliogenesis and cell cycle progression. Disruption of ODF2 impairs centriolar satellite assembly, leading to defective centrosome organization and compromised primary cilium formation.
In the Raji Burkitt lymphoma background, ODF2 knockout permits dissection of centrosome dynamics in a malignancy frequently exhibiting centrosome amplification and genomic instability. Although lymphocytes lack primary cilia, centrosome functions in mitotic progression and signaling remain critical. This model links ODF2 loss to potential defects in spindle organization, cell cycle control, and ciliopathy-like phenotypes in a cancer context, offering a tool to explore centrosome-targeted vulnerabilities.
Typical applications include immunofluorescence and ciliogenesis induction assays to assess primary cilium assembly, western blotting and RT-qPCR for expression analysis, and flow cytometry for cell cycle profiling. Co-immunoprecipitation studies identify ODF2 interaction networks, while centrosome amplification assays probe cancer cell biology. This knockout population also supports infertility research by modeling dysfunction of sperm tail outer dense fibers. For further technical information, please contact Ascent Research.
