The OSBPL11 Knockout Raji Polyclonal Cells comprise a CRISPR/Cas9-edited polyclonal knockout population of Raji B lymphoblastoid cells, engineered to disrupt the OSBPL11 gene. This polyclonal format provides a heterogeneous pool of edited cells for immediate use in loss-of-function studies, eliminating the time-intensive steps of single-cell cloning and expansion. The product offers a powerful tool for investigating oxysterol-binding protein-like 11 function in a lymphoma-relevant background.
Raji cells are an EBV-positive human B lymphoblastoid line originally isolated from an 11-year-old male with Burkitt lymphoma. They grow in suspension, express canonical B-cell markers (CD19, CD20, CD22), and retain key signaling properties of malignant B lymphocytes. Widely adopted in immunology and cancer research, Raji cells serve as a robust model for studying B-cell receptor signaling, viral oncogenesis, and lymphomagenesis.
OSBPL11 is a member of the oxysterol-binding protein-related protein family that functions at endoplasmic reticulum?Cplasma membrane contact sites. It facilitates non-vesicular transfer of cholesterol and phosphatidylinositol 4-phosphate (PI4P), acting as a molecular bridge through direct interactions with VAPA and VAPB. OSBPL11 expression is under transcriptional control of sterol regulatory element-binding proteins (SREBPs) and liver X receptor (LXR), integrating oxysterol signals to modulate lipid distribution. Downstream, OSBPL11 influences the organization of cholesterol-rich lipid rafts, Rho GTPase signaling components, and the phosphorylation status of AKT, a central kinase in growth and survival pathways. Within the broader lipid-exchange network, OSBPL11 cooperates with OSBP, CERT, PITP, and Sac1 to orchestrate membrane lipid composition and signaling platform assembly.
In Raji lymphoma cells, disrupting OSBPL11 compromises cholesterol trafficking and alters plasma membrane lipid architecture, which likely perturbs B-cell receptor-mediated signaling and proliferative capacity. Because lymphoblastoid cells depend on precise lipid microdomain organization for signal transduction, this knockout model enables dissection of how oxysterol-regulated lipid transport shapes malignant B-cell phenotypes. It also provides an isogenic platform to explore dependencies on sterol homeostasis that may be therapeutically exploitable in B-cell malignancies.
Researchers can utilize this polyclonal knockout population in a variety of assays, including Western blotting and RT-qPCR for gene and protein expression analysis, immunofluorescence and Filipin staining to visualize cholesterol distribution, flow cytometry for apoptosis and cell cycle profiling, and drug sensitivity screens. Transcriptomic approaches such as RNA-seq and interaction studies via co-immunoprecipitation of VAPA or VAPB further extend the model??s utility. For additional details and ordering, please contact Ascent Research.
