The PABPC4 Knockout Raji Polyclonal Cells consist of a CRISPR/Cas9-edited polyclonal population of Raji B lymphoblastoid cells carrying targeted disruptions in the PABPC4 gene. This loss-of-function model enables the investigation of cytoplasmic poly(A)-binding protein 4 in human B lymphocyte biology without requiring clonal isolation. The polyclonal format captures diverse editing outcomes, representing a heterogeneous knockout pool suitable for functional studies.
Raji cells are derived from an EBV-positive Burkitt’s lymphoma and serve as a canonical model for B cell malignancy. Characterized by constitutive MYC overexpression and active mTORC1 signaling, these cells are extensively used in cancer, immunology, and virology research to study lymphomagenesis and viral host interactions.
PABPC4 is a cytoplasmic poly(A)-binding protein that specifically interacts with the 3′ poly(A) tail of mature mRNAs. Through direct binding to translation initiation factor eIF4G and scaffold proteins PAIP1 and PAIP2, PABPC4 facilitates mRNA circularization by bridging the 5′ cap-associated eIF4F complex to the poly(A) tail. This closed-loop structure enhances ribosome recruitment and translation initiation efficiency. PABPC4 activity is regulated by the MYC transcription factor and mTORC1 signaling, and it functions downstream of eIF4E-mediated cap-dependent translation activation. Additionally, PABPC4 plays a role in nonsense-mediated mRNA decay, coupling translation to mRNA surveillance. Disruption of PABPC4 impairs poly(A)-dependent translation, potentially diminishing synthesis of short-lived regulatory proteins.
In the Raji Burkitt’s lymphoma model, high MYC levels and EBV latency reprogram gene expression to sustain proliferation and immune evasion. PABPC4 likely supports this oncogenic state by promoting the efficient translation of mRNAs encoding growth-promoting and viral factors. Knockout of PABPC4 in these polyclonal cells is expected to compromise ribosome loading on polyadenylated transcripts, altering the proteomic output and offering a tool to interrogate the role of cytoplasmic poly(A)-binding proteins in lymphomagenesis and antiviral responses.
Researchers can employ these cells in a range of assays: western blotting and RT-qPCR for confirming knockout and transcriptional effects, polysome profiling to measure translation efficiency, RNA immunoprecipitation for protein?CRNA interaction mapping, flow cytometry for phenotypic analysis, and RNA-seq for global expression studies. Typical applications include mRNA translation research, B cell lymphoma mechanistic studies, RNA-binding protein characterization, and drug target validation. For technical inquiries or to request a quotation, please contact Ascent Research.
