The PAK4 Knockout Raji Polyclonal Cells comprise a CRISPR/Cas9-edited polyclonal knockout cell population with targeted disruption of the PAK4 gene in Raji B lymphocytes. This mixed population provides a loss-of-function model for studying PAK4-dependent mechanisms without clonal selection artifacts, and is well-suited for pooled functional screens and robust signaling analyses.
Raji cells are an EBV-positive B lymphoblastoid line derived from a Burkitt lymphoma patient, widely used as a model for B-cell malignancies. They retain characteristics of mature B lymphocytes and support investigation of B-cell receptor signaling, lymphomagenesis, and therapeutic responses, making them an ideal host for gene-editing studies.
PAK4 is a serine/threonine kinase acting downstream of the Rho GTPases Cdc42 and Rac1, which are activated by upstream signals such as HGF, EGF, and integrin ligation. PAK4 phosphorylates key substrates including LIMK1, cofilin, ??-catenin, and Bad, regulating cytoskeletal dynamics, cell migration, proliferation, and survival. It also phosphorylates transcription factors CREB and Smad2/3 and drives Wnt/??-catenin-mediated transcription of cyclin D1 and c-Myc. The kinase cross-talks with MAPK/ERK and PI3K/AKT pathways and interacts with scaffold proteins PIX, GIT1, and ILK to localize signaling.
In Raji cells, PAK4 knockout provides a physiologically relevant system to examine its roles in B-cell lymphomagenesis and therapy resistance. Disruption of PAK4 in this EBV-immortalized background allows dissection of its contributions to B-cell receptor downstream cascades, NF-??B activation, and survival programs. The model also facilitates study of integrin-mediated adhesion and migration pathways that influence lymphoma dissemination.
Applications include Western blot detection of phosphorylated LIMK1, ERK, and ??-catenin; proliferation assays (MTS, EdU); Transwell migration/invasion; apoptosis assays (annexin V); cytoskeletal staining; TCF/LEF reporter assays; and RT-qPCR of target genes. This polyclonal knockout product is also amenable to functional genomics screening and drug-resistance profiling. For additional information or to discuss your project, please contact Ascent Research.
