The PDZD11 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population that eliminates PDZD11 expression in the Raji human B lymphocyte line. This polyclonal pool was generated via CRISPR/Cas9-mediated gene disruption, offering a robust loss-of-function model for studying PDZD11-dependent pathways without clonal selection biases. The mixed population is ideal for high-throughput functional screens and pooled phenotypic assays, enabling systematic interrogation of PDZD11??s role in lymphocyte adhesion and junctional complex dynamics.
Raji cells are a widely used B lymphocyte model derived from a Burkitt??s lymphoma patient. They exhibit a mature B-cell phenotype, constitutive proliferation driven by MYC oncogene translocations, and the capacity for inducible adhesion and migration upon integrin stimulation. These characteristics make Raji cells a pertinent host for investigating adhesion-dependent signaling events in B-cell lymphoma, including transendothelial migration and tumor-stroma interactions.
PDZD11 serves as a molecular scaffold that links nectin family receptors and PLEKHA7 to the actin cytoskeleton at apical adherens junctions. It interacts directly with AFADIN, nectins, and actin filaments, and is activated downstream of integrin and nectin receptor engagement. PDZD11-mediated complexes organize actin cytoskeleton remodeling, Rho GTPase signaling, and apical polarity maintenance; its disruption leads to compromised junctional integrity, altered cell polarity, and dysregulated adhesion signaling.
In the lymphocytic Raji model, which lacks classical epithelial junctions, PDZD11 may play an analogous role in organizing adhesion complexes that govern B-cell migration, invasion, and interactions with the microenvironment. Knockout of PDZD11 allows researchers to dissect its non-epithelial functions, such as potential contributions to lymphocyte homing, transendothelial trafficking, and adhesion-mediated survival signaling, providing insight into lymphoma dissemination mechanisms.
Key research applications include B-cell adhesion assays, lymphoma invasion studies, and investigation of apical junction complex biology in hematopoietic contexts. This product supports diverse assays: Western blotting for PDZD11, PLEKHA7, and AFADIN; immunofluorescence for junctional proteins; fibronectin adhesion assays; Transwell migration and invasion experiments; co-immunoprecipitation of PDZD11-containing complexes; flow cytometry for surface adhesion molecules; and RNA-seq transcriptomic profiling. For additional information, please contact Ascent Research.
