Ptbp1 Knockout NIH 3T3 Cell Line

Product Type:
Genome-edited Cells
Disease:
Normal
Gene Species:
Mus musculus (Mouse)

Datasheet

The Ptbp1 Knockout NIH 3T3 Cell Line is a CRISPR/Cas9-edited knockout cell line derived from mouse embryonic fibroblasts, designed for the study of alternative splicing and cellular reprogramming. Ptbp1 encodes an RNA-binding protein that represses neuron-specific exon inclusion; its disruption allows the investigation of splicing switches and neurogenic conversion in a well-characterized fibroblast model.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

NIH 3T3

Gene Name

Ptbp1

Gene Species

Mus musculus (Mouse)

Gene Identifier

NCBI Gene ID 19205
Culture Conditions

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

Daily monitoring confirms that the cells are free from bacterial, yeast, and fungal contamination.

Mycoplasma testing

Negative for mycoplasma through PCR analysis

Pathogens

Cells tested negative for HIV-1, HBV, and HCV.
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The Ptbp1 Knockout NIH 3T3 Cell Line is a CRISPR/Cas9-edited knockout cell line derived from immortalized mouse embryonic fibroblasts, providing a stable loss-of-function model for the Ptbp1 gene. This cell line enables researchers to investigate the functional consequences of Ptbp1 disruption in a well-characterized fibroblast background. As a knockout cell line, it offers a homogeneous population for reproducible studies, avoiding the variability inherent in transient knockdown approaches. The gene editing was performed using CRISPR/Cas9 technology to disrupt the Ptbp1 locus, eliminating expression of the encoded RNA-binding protein. This model is ideal for controlled experiments on alternative splicing regulation, cellular reprogramming, and transdifferentiation, where Ptbp1 acts as a critical repressor of neuron-specific exon inclusion.