CIRBP Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji human B lymphoblast cell line. This product enables targeted disruption of the CIRBP gene, generating a heterogeneous pool of knockout cells suitable for loss-of-function analyses. The polyclonal format minimizes artifacts from clonal selection while maintaining biological complexity, supporting robust mechanistic studies.
The Raji cell line, established from a Burkitt’s lymphoma patient, is an EBV-positive B lymphoblast model widely employed in B cell biology and lymphoma research. These cells exhibit antibody-producing characteristics and are instrumental for investigating immune surveillance and oncogenic processes. Their EBV-positive lymphoblastoid phenotype makes them particularly pertinent for examining stress responses and survival signaling intrinsic to B cell malignancies.
CIRBP encodes cold-inducible RNA-binding protein, an RNA chaperone that modulates post-transcriptional gene regulation under cellular stress. Induced by cold shock, hypoxia, UV radiation, and TNF-??, CIRBP interacts with hnRNP A1, eIF4G, and NF-??B p65, and associates with RNA granule components. It regulates downstream targets including cyclin D1, RPA2, TRX, and DUSP6, thereby influencing MAPK/ERK signaling, NF-??B pathways, circadian rhythm, and apoptosis modulation.
In the Raji B lymphoblast context, CIRBP disruption likely abrogates stress-induced stabilization of target mRNAs, perturbing cell cycle and pro-survival protein expression. This may sensitize cells to apoptosis under genotoxic or hypoxic stress, offering a model to explore stress adaptation mechanisms in lymphoma. The polyclonal knockout allows interrogation of CIRBP-mediated integration of stress signals via NF-??B and MAPK/ERK cascades, potentially revealing lymphoma-specific vulnerabilities.
This knockout model supports diverse applications including functional dissection of CIRBP in B cell lymphoma, synthetic lethal screening, and drug sensitivity assessment under altered stress responses. Representative assay techniques include Western blotting, RT-qPCR, RNA-seq, flow cytometry for apoptosis and proliferation, cold shock/hypoxia stress assays, immunofluorescence, and co-immunoprecipitation. These polyclonal cells advance research in cancer biology, inflammatory disorders, neurodegenerative diseases, and hypoxia-associated pathologies. For additional information, please contact Ascent Research.
