COPS7A Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population in which the COPS7A gene has been disrupted. This loss-of-function model provides a pooled Raji cell background for studying the COP9 signalosome (CSN) without requiring clonal isolation. The polyclonal format enables robust population-based assays and screening campaigns.
The Raji host cell line originates from a human Burkitt??s lymphoma, is Epstein-Barr virus (EBV) positive, and expresses B lymphocyte surface markers. Raji cells serve as a well-established model for B cell biology, lymphomagenesis, and immune response studies, and their EBV positivity offers additional relevance for viral oncology research.
COPS7A is a core subunit of the COP9 signalosome, which catalyzes the deneddylation of cullin-RING E3 ubiquitin ligases (CRLs). By removing NEDD8 from cullin scaffolds such as CUL1, CUL3, and CUL4, the CSN inactivates CRLs and stabilizes substrates including p53, ??-catenin, and cyclin E. This regulatory node connects protein ubiquitination and degradation to JAK-STAT, NF-??B, and MAPK/ERK signaling pathways. COPS7A interacts with other CSN subunits (COPS1?C8), CUL1-Rbx1, NEDD8, and USP15, and its activity is modulated by CDK/cyclin kinases, NEDD8 conjugation machinery, and DNA damage signals.
Disruption of COPS7A in Raji B lymphocytes alters CRL-mediated ubiquitination, likely affecting cell cycle progression, apoptosis, and NF-??B-driven survival programs. This perturbation creates a valuable system for dissecting the role of the ubiquitin-proteasome system in Burkitt??s lymphoma, especially in the context of EBV-driven oncogenesis, and for identifying vulnerabilities to proteasome inhibitors.
Key applications include functional analysis of the CSN/CRL axis, mechanistic investigation of ubiquitin-dependent signaling in B cell malignancies, and drug screening for proteasome-modulating agents. Researchers can employ ubiquitination assays, Western blotting for substrate stabilization (e.g., p53, ??-catenin), deneddylation activity assays, flow cytometry for cell cycle and apoptosis, co-immunoprecipitation of CSN complexes, and RNA-seq-based transcriptomics. For further experimental guidance or additional product details, contact Ascent Research.
