The CORO1C Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human Raji B lymphocyte cell line. This product provides a loss-of-function model for the CORO1C gene, enabling investigation of its roles in actin cytoskeleton remodeling. The polyclonal format preserves population-level heterogeneity while disrupting the target gene through CRISPR/Cas9-mediated gene disruption, offering a versatile tool for studying CORO1C-dependent phenotypes in a lymphoblastoid context.
The Raji cell line is an Epstein-Barr virus (EBV)-immortalized human B lymphocyte line originally isolated from a patient with Burkitt lymphoma. These lymphoblastoid cells retain key characteristics of B lymphocytes, including surface immunoglobulin expression and the capacity for immune synapse formation. Their robust growth and well-characterized signaling networks make them a widely used model for lymphoma biology, immune cell function, and cytoskeletal dynamics.
CORO1C (Coronin 1C) is an actin-binding protein that critically regulates actin filament dynamics through interactions with the Arp2/3 complex and F-actin. Activated downstream of Rac1 and Cdc42, CORO1C promotes lamellipodia formation and cell migration by modulating Arp2/3-dependent actin branching. It also interacts with cofilin, cortactin, vinculin, talin, and integrins, linking extracellular adhesion signals to the actin cytoskeleton. This positions CORO1C as a key node in pathways governing cell polarity, endocytosis, and focal adhesion turnover.
In Raji B cells, CORO1C knockout impairs actin-dependent processes such as adhesion, migration, and membrane trafficking. Given the role of B lymphocytes in immune surveillance and the metastatic potential of lymphoma cells, disrupting CORO1C provides insights into how actin remodeling influences immune synapse assembly, chemotaxis, and tissue invasion. The model is particularly relevant for dissecting mechanisms that drive lymphoma dissemination and for identifying vulnerabilities in actin regulatory networks that could be targeted to limit metastasis.
This polyclonal knockout cell pool is suitable for a range of functional assays, including Transwell migration assays to measure cell motility, immunofluorescence staining to visualize actin structures, and flow cytometry to assess surface receptor dynamics. Western blotting confirms CORO1C protein loss, while adhesion and Rho GTPase activation assays evaluate integrin-mediated signaling. These cells are ideal for screening anti-metastatic compounds, studying B cell migration in chemokine gradients, and exploring CORO1C’s role in immune cell function. For further details or custom inquiries, please contact Ascent Research.
