COTL1 Knockout Raji Polyclonal Cells represent a CRISPR/Cas9-mediated gene-disrupted polyclonal population of the human B lymphocyte cell line Raji, engineered to ablate expression of the COTL1 gene. This knockout model introduces targeted disruption of the COTL1 locus, generating a mixed population of cells with loss-of-function modifications. The polyclonal pool retains genetic heterogeneity, providing a robust system for studying gene function in a lymphoma context.
The Raji host cell line, derived from a Burkitt??s lymphoma patient, is an Epstein?CBarr virus (EBV)-positive B lymphocyte line widely employed in immunology and cancer research. Raji cells serve as a model for antibody production and humoral immunity, expressing characteristic B-cell surface markers and exhibiting functional immunoglobulin secretion. Their transformed phenotype and established use in drug screening and signal transduction studies make them particularly suitable for assessing the role of COTL1 in B-cell biology and leukotriene-mediated inflammation.
COTL1 (coactosin-like protein 1) functions as an actin-binding scaffold that directly links 5-lipoxygenase (ALOX5) to the F-actin cytoskeleton, thereby controlling leukotriene biosynthesis and actin reorganization. Upon stimulation by upstream regulators including PMA, calcium ionophores, and inflammatory cytokines IL-4 and IL-13, COTL1 promotes ALOX5 activation and synthesis of leukotriene B4. The protein physically interacts with ALOX5, F-actin, and cofilin (CFL1), facilitating cofilin-mediated actin depolymerization and driving cell migration. Key downstream pathway components include 5-lipoxygenase-activating protein (FLAP), leukotriene A4 hydrolase (LTA4H), and leukotriene C4 synthase (LTC4S), collectively linking arachidonic acid metabolism to dynamic cytoskeletal changes.
In Raji B lymphocytes, COTL1-dependent regulation of leukotriene production and actin dynamics is anticipated to influence cell shape, adhesion, and chemotactic migration??functions essential for B-cell homing and immune responses. Disruption of COTL1 in this EBV-positive lymphoma background provides a loss-of-function model for delineating the crosstalk between arachidonic acid metabolism and cytoskeletal reorganization. This knockout pool is thus relevant to studies of leukotriene-driven inflammation, B-cell malignancy metastasis, and pathologies such as asthma and other leukotriene-related disorders.
The COTL1 Knockout Raji Polyclonal Cells can be employed in a range of functional assays, including Western blotting and RT-qPCR for confirming gene disruption, leukotriene B4 ELISA for quantifying eicosanoid output, actin polymerization assays to monitor cytoskeletal dynamics, and Transwell or flow cytometry-based migration assays to assess cell motility. These cells are suitable for drug screening targeting 5-lipoxygenase or its regulators, investigation of B-cell signaling networks, and mechanistic studies of cancer cell migration. For further technical details, please contact Ascent Research.
