The DAAM1 Knockout Raji Polyclonal Cells constitute a CRISPR/Cas9-mediated gene-disruption model in the human Raji B lymphocyte cell line. This polyclonal knockout cell population carries targeted disruption of the DAAM1 gene, providing a heterogeneous loss-of-function system to interrogate DAAM1-dependent actin dynamics and Wnt/PCP signaling. The use of polyclonal knockout cells eliminates clonal selection artifacts and enables robust analysis of DAAM1 biology across a pool of edited cells, suitable for functional assays that require population-level responses.
The Raji cell line is a well-established human EBV-positive Burkitt lymphoma model derived from B lymphocyte lineage. These suspension-adapted cells are widely employed in B-cell malignancy research, immunological signaling studies, and hematopoietic cancer drug screening. Their rapid proliferation, genetic stability, and well-characterized signal transduction networks make them an ideal host for CRISPR-based knockout studies, particularly for dissecting oncogenic pathways that intersect with cytoskeletal remodeling and cell polarity.
DAAM1 (Dishevelled-associated activator of morphogenesis 1) encodes a formin protein that functions as a critical actin nucleator downstream of the non-canonical Wnt/PCP pathway. Upon Wnt5a-Frizzled receptor activation, DAAM1 binds to Dishevelled (DVL1/2/3) and the Rho GTPases RhoA, Rac1, and Cdc42 to promote unbranched actin filament elongation and the formation of stress fibers and filopodia. DAAM1 also interacts with profilin to regulate actin monomer delivery and participates in feedback regulation of RhoA, linking cytoskeletal dynamics to transcriptional responses via ROCK, JNK, and c-Jun. Additionally, DAAM1 modulates ??-catenin signaling, bridging Wnt pathway branches.
In Raji B lymphocytes, DAAM1 contributes to cell migration, polarity, and proliferation??processes frequently dysregulated in lymphoma. Disruption of DAAM1 in this model is expected to impair Wnt/PCP-driven cytoskeletal rearrangements, leading to reduced filopodia formation, altered Rho GTPase activity, and diminished migratory capacity. This knockout system thus provides a relevant cellular context to study DAAM1??s role in B-cell malignancy progression, immune synapse formation, and potential contributions to neurodevelopmental disorder mechanisms through conserved actin regulatory functions.
The DAAM1 Knockout Raji Polyclonal Cells are well-suited for a range of research applications, including Wnt/PCP pathway dissection, actin cytoskeleton characterization, and lymphoma pathogenesis investigation. Users can assess DAAM1 protein levels and actin structures via Western blotting and immunofluorescence, probe protein?Cprotein interactions by co-immunoprecipitation, and evaluate migration and proliferation using standard assays. Transcriptomic and phenotypic profiling through RNA-seq and flow cytometry, along with Rho activity measurements, further enable comprehensive pathway analysis and drug target validation. For further information, please contact Ascent Research.
