The FUT8 Knockout Raji Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal population derived from human Raji B lymphoblastoid cells, with targeted disruption of FUT8. This model enables investigation of core fucosylation-dependent glycoprotein functions in B lymphocytes. The polyclonal format captures diverse knockout alleles, facilitating robust functional studies without clonal expansion. By eliminating FUT8 activity, these cells offer a definitive loss-of-function system for analyzing fucosylation-specific effects on receptor signaling, antibody effector functions, and immune synapse formation.
The Raji cell line is an EBV-positive Burkitt??s lymphoma-derived B lymphoblastoid line that retains mature B cell characteristics, including surface immunoglobulin expression, antigen presentation, and cytokine secretion. It is extensively used in immunology and oncology for studying B cell activation, antibody production, and immune effector functions. Its transformed nature allows easy culture and genetic manipulation while maintaining Fc receptor expression and ADCC capability, making it an ideal host for examining FUT8-dependent glycosylation in B cells.
FUT8 encodes ??1,6-fucosyltransferase, which catalyzes core fucosylation??the transfer of fucose from GDP-fucose to the innermost GlcNAc of N-glycans. This modification stabilizes glycoproteins and modulates signaling of EGFR and TGFBR1, while reducing IgG Fc binding to Fc?? receptors, thereby attenuating ADCC. FUT8 expression is regulated by SP1 and TGF-??, and its activity requires GDP-fucose and acceptor N-glycans. Downstream, core fucosylation influences integrin-mediated adhesion, immune synapse formation, and downstream pathways involving phospho-EGFR and TGF-??.
In Raji B cells, FUT8 knockout globally eliminates core fucosylation, disrupting glycoprotein-dependent functions. This directly impacts ADCC, as B cell-produced antibodies or target cell-bound IgG exhibit altered Fc?? receptor engagement. Additionally, impaired fucosylation of TGFBR and EGFR likely attenuates downstream signaling, affecting proliferation, apoptosis, and cytokine secretion. The model thus allows dissection of core fucose roles in B lymphocyte biology, antibody effector mechanisms, and glycosylation perturbations in B-cell malignancies like Burkitt??s lymphoma.
This polyclonal knockout product is applicable in glycobiology, cancer immunotherapy, and ADCC research. Typical assays include LCA lectin blot for core fucose, flow cytometry for fucosylated surface markers, ADCC reporter assays, RT-qPCR for glycosylation enzymes, mass spectrometry glycomics, and phospho-EGFR/TGFBR analysis. The cells enable functional studies of B cell biology, antibody production, and immune synapse dynamics. For inquiries, contact Ascent Research.
