GDAP1 Knockout Raji Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Bone
Disease:
Burkitt lymphoma

Datasheet

CRISPR/Cas9-edited polyclonal knockout of GDAP1 in Raji B lymphocytes, designed for investigating mitochondrial fission, apoptosis, and Charcot-Marie-Tooth disease mechanisms. GDAP1, a mitochondrial outer membrane protein, interacts with DRP1 and FIS1 to promote mitochondrial division, and its disruption leads to elongated mitochondria and neuronal dysfunction. This knockout model enables detailed analysis of mitochondrial morphology, apoptotic signaling, and oxidative stress pathways in an EBV-positive Burkitt's lymphoma background, supporting drug screening and mechanistic studies in peripheral neuropathy.

Inquire Now

In stock

Ships next business day

Ask a Question

Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

Raji

Cell Type

B cell line

Sex of Donor

Male

Age

11 years

Derived From Site

In situ; Maxilla

Gene Name

GDAP1

Gene Identifier

NCBI Gene ID 54332

Morphology

Lymphoblast-like

Growth Mode

Suspension

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The GDAP1 Knockout Raji Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population with targeted disruption of the GDAP1 gene, generated in the Raji B lymphocyte cell line.

This polyclonal knockout model provides a heterogeneous pool of gene-edited cells, allowing researchers to study the loss-of-function effects of GDAP1 without the constraints of monoclonal selection, thereby capturing a broader range of phenotypes relevant to mitochondrial dynamics and peripheral nerve pathology.