MOCOS Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population of Raji B lymphocytes with targeted disruption of the MOCOS gene. This loss-of-function model enables investigation of molybdenum cofactor sulfurase biology in a lymphoblastoid context without clonal selection. The Raji cell line, derived from Burkitt’s lymphoma, is a suspension-adapted, EBV-positive line expressing B-cell markers CD19 and CD20, widely used in immunology and cancer research.
Raji cells serve as a robust model for B-cell function, exhibiting antigen presentation capabilities and antibody production. Their genetic stability and ease of manipulation make them ideal for CRISPR/Cas9-mediated gene disruption and subsequent phenotypic analyses. Key features include suspension growth, expression of hallmark B-cell receptors, and retention of signaling pathways relevant to lymphomagenesis.
The MOCOS protein catalyzes the sulfurylation of the molybdenum cofactor (MoCo), an essential step for the activation of xanthine oxidase and aldehyde oxidase. Activated xanthine oxidase drives the conversion of hypoxanthine to xanthine and xanthine to uric acid in purine catabolism, generating superoxide. Aldehyde oxidase mediates xenobiotic oxidation. Interacting factors include xanthine dehydrogenase and aldehyde oxidase 1. Regulation of MOCOS in B cells may involve the transcription factor PAX5.
Disruption of MOCOS in Raji cells impairs MoCo-dependent oxidases, leading to accumulation of purine intermediates and reduced uric acid production. This metabolic shift affects nucleotide balance and redox homeostasis, potentially altering cell proliferation and drug sensitivity. The model is relevant to type II xanthinuria and for studying the role of purine metabolism and aldehyde oxidase activity in B-cell lymphoma drug resistance and toxicity.
Typical applications include Western blotting and RT-qPCR for MOCOS expression analysis, xanthine oxidase activity assays, and uric acid quantification. Drug sensitivity profiling with aldehyde oxidase substrates, flow cytometry for B-cell markers, and cell proliferation assays are also facilitated. For additional information, please contact Ascent Research.
