NANS Knockout Raji Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Bone
Disease:
Burkitt lymphoma

Datasheet

CRISPR/Cas9-edited polyclonal NANS knockout Raji cells, a Burkitt??s lymphoma-derived B lymphocyte model, enable dissection of sialic acid biosynthesis and glycoimmunology. NANS disruption blocks conversion of N-acetylmannosamine 6-phosphate to N-acetylneuraminate 9-phosphate, impairing sialylation of glycoconjugates downstream of GNE and upstream of sialyltransferases such as ST6GAL1. Ideal for studying congenital disorders of glycosylation, B cell biology, and cancer glycosylation through lectin-based flow cytometry, sialic acid quantification, and cell adhesion assays.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

Raji

Cell Type

B cell line

Sex of Donor

Male

Age

11 years

Derived From Site

In situ; Maxilla

Gene Name

NANS

Gene Identifier

NCBI Gene ID 54187

Morphology

Lymphoblast-like

Growth Mode

Suspension

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

NANS Knockout Raji Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal cell population in which the NANS gene, encoding N-acetylneuraminic acid synthase, has been disrupted.

This knockout model provides a powerful loss-of-function tool for investigating sialic acid biosynthesis and its downstream effects on glycosylation-dependent processes.

The polyclonal format ensures a broad representation of editing outcomes across the population, avoiding single-clone bias and enabling robust phenotypic screening in a physiologically relevant immune cell background.