OTUD7B Knockout AGS Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Stomach
Disease:
Adenocarcinoma

Datasheet

The OTUD7B Knockout AGS Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population of human gastric adenocarcinoma cells (AGS) with targeted disruption of the OTUD7B gene, a K63-linked deubiquitinase that negatively regulates NF-??B signaling. OTUD7B removes K63-linked polyubiquitin chains from substrates such as TRAF6 and RIP1, thereby limiting pathway activation. This knockout model enables sustained NF-??B signaling in a gastric epithelial context, making it valuable for investigating gastric cancer biology, inflammation, ubiquitin-mediated regulation, and drug sensitivity. Key applications include western blotting, NF-??B reporter assays, and functional studies of cell survival and migration.

Inquire Now

In stock

Ships next business day

Ask a Question

Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

AGS

Sex of Donor

Female

Age

54 years

Derived From Site

In situ; Stomach

Gene Name

OTUD7B

Gene Identifier

NCBI Gene ID 56957

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

Ham's F-12

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The OTUD7B Knockout AGS Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal population of human gastric epithelial adenocarcinoma cells harboring a targeted disruption of the OTUD7B gene.

This loss-of-function model is designed for the study of OTUD7B-dependent negative regulation of NF-??B signaling in a gastric cancer-relevant cellular background.