The PALM Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population for disrupting PALM gene function in the Raji B lymphocyte line. This heterogeneous pool provides a loss-of-function model free of clonal biases, enabling consistent study of PALM-dependent membrane organization, adhesion, and migration. The polyclonal format retains genetic diversity, suitable for analyses where variable lipid raft or adhesion receptor expression affects cellular behavior.
The parental Raji cell line, derived from EBV-positive Burkitt lymphoma, is a widely used B lymphocyte model featuring antibody production, antigen presentation, and robust integrin signaling. Raji cells express surface immunoglobulin, functional lipid rafts, and dynamic actin remodeling, making them ideal for investigating PALM-mediated processes. The EBV-driven proliferation also provides an oncogenic context to examine how adhesion and motility alterations contribute to lymphoma progression.
PALM (paralemmin) is a palmitoylated lipid raft protein that scaffolds plasma membrane organization and links rafts to the actin cytoskeleton via interactions with actin, flotillins, and caveolin-1. Regulated by palmitoyltransferases and integrin engagement, PALM modulates focal adhesion turnover through FAK, Src, RhoA, and Rac1, driving actin reorganization, cell spreading, and migration. By integrating cholesterol and PIP2 signals, PALM controls adhesion complex dynamics; its disruption impairs cell motility and membrane architecture.
In Raji B cells, PALM knockout disrupts lipid raft integrity and integrin-mediated adhesion critical for B cell trafficking, antigen encounter, and immune synapse formation. Raji cells require dynamic actin and adhesion for transendothelial migration and stromal interactions, processes directly regulated by PALM. Loss of PALM thus attenuates adhesion and spreading, providing a model for investigating B cell motility defects in lymphoma dissemination and other disorders.
Applications include B cell adhesion and migration assays, lipid raft signaling studies, and cytoskeletal organization research. Techniques such as Western blotting, flow cytometry, and immunofluorescence confirm PALM disruption and phenotype changes; co-immunoprecipitation and RNA-seq dissect interactomes and transcriptomes. Further applications encompass live-cell migration tracking and pharmacological perturbation studies. This polyclonal knockout model offers a versatile system for dissecting PALM-dependent mechanisms in B cell biology and lymphoma. For comprehensive product characterization data and support, please contact Ascent Research.
